SAMEA114472018 - Seeds from individual SSD lines were grown in a climatic chamber in 96-well trays for two weeks. Two leaf segments (each approximately 3 cm in length ) were placed in a 96-well polypropylene cluster tube (1.2 ml, 8 tube stripe format Corning) containing one 2 mm lead bead. The tubes were closed with 8-cap tube strips. Samples were frozen in liquid nitrogen and stored at -80°C until use. Materials were grinded by Tissue Lizer (Quigen) and DNA was extracted with MATAB, cleaned with chloroform-isoamyl-alcohol 24:1, precipitated with isopropanol and washes with 70% Ethanol. After resuspending in Tris 10mM pH 8.4, DNAs were treated with RNAse A pancreatic, quantified by Qubit and checked for integrity by agarose gel electrophoresis. | seqout