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SAMEA11301992 - Seeds were provided by CHE001. Individual plants for DNA extraction were grown in a climate chamber at UZH. Two segments of the first leaf (each approximately 3 cm in length and 0.3 to 0.5 cm wide) were placed in 2.2 ml tubes together with two 4mm glass beads (ROTH). Samples were frozen in liquid nitrogen and grinded with a Geno/Grinder (SPEX SamplePrep) at 1500 rpm for 1 minute. DNA was extracted with Serapure magnetic beads according to Baptiste Mayjonade et al., Extraction of high-molecular-weight genomic DNA for the long-read sequencing Biotechniques, 2016. Purified DNA was disolved in 10mM Tris HCl pH 8.0 and shipped frozen to IHAR with blue ice. | seqout