ERS24517746 - For each accession, 100 seeds were randomly selected and germinated under controlled conditions 11 h light at 22°C / 13 h dark at 15°C in a GS10/11 chamber Flohr Instruments, NL. Cotyledons were harvested from 50 seedlings per accession, pooled, frozen at −18°C, and lyophilised for 24 hours Alpha 1-4 LD plus, Martin Christ, Germany. For non-genebank samples outgroups, material was collected from a private garden. | seqout